ColiMinder™ is an analyser for monitoring fecal contamination in surface water.
It is able to detect microbiological contamination in 15 minutes.
How does it work?
The ColiMinder measures the specific metabolic (enzymatic) activity of the target organisms present in the sample.
The measured value provides information about the energy turnover of living organisms in the sample. Living organisms need energy, which they gain through their metabolism, so the metabolic activity reflects the energetic turnover of the existing microorganisms.
The measured activity directly indicates the faecal contamination level of the water.
How do I Use the ColiMinder?
ColiMinder can automatically monitor any type of surface water, bathing and recreational water and raw water, be it fresh or saline water, providing a direct visualization of microbial dynamics of the respective water body.
The timeline displays any change in microbial contamination, providing precise and valuable information that was previously unavailable.
Since the results of the ColiMinder are available in near real time, microbiological water quality is now available as an evidence-based decision parameter. In addition, real-time alerts can be sent when defined bacterial contamination limits are exceeded.
The ColiMinder has proven its ability to specifically measure any type of fecal indicator bacteria or total microbial activity in surface water. It reliably detects any type of contamination, whether it is of fecal or microbial origin.
Different add-on modules are available to ensure stable and reliable sampling and results, even in harsh environmental conditions.
How do I use the results?
The measured enzymatic activity of the sample provides a direct answer to the question: “how high is the faecal contamination of the sample?”.
Depending on the reagent used, the measurement results are specific for the corresponding target organism.
The results not only provide direct quantitative information about the microbial contamination of the product, but shape and course of the measurement timeline also contain valuable information about the dynamics of the microbiological load and thus allow conclusions to be drawn about the causes of changes. Depending on the reagent used, the measurement results are specific for the corresponding target organism.
The analytical capability of ColiMinder in surface water measurements is comparable to that of determinations of HPC or to that of chromogenic coliform agar counts in the case of fecal contamination.
In many different scientific studies the ColiMinder has been used, evaluated or validated for surface and raw water monitoring, e.g. in the latest publication by Burnet, J.-B et.al (2021). Automated Targeted Sampling of Waterborne Pathogens and Microbial Source Tracking Markers Using
Near-Real Time Monitoring of Microbiological Water Quality. Water, 13, 2069.
The reliability of the ColiMinder has been demonstrated in several installations where the devices have been providing reliable measurement results in continuous operation since 2015.
Thanks to the sophisticated system for fully automatic cleaning and calibration, the maintenance effort is limited to refilling the consumables. Replacement of the silicone hoses is recommended every 2 years, otherwise no maintenance is required.
The ColiMinder instruments measure absolutely stable, no manual calibration is necessary as the instruments calibrate themselves automatically at regular intervals.
The ColiMinder can measure any type of sample without pre-treatment. It has no problem with suspended sediments or turbid samples.
Even fluctuating background fluorescence has no effect on the measurement result due to the measurement approach.
Its robustness towards environmental influences, like temperature, humidity, chemical load etc., is evident in installations like at Stonecutter Island Sewage Treatment Plant in Hong Kong, where it has been monitoring effluent 24/7 since 2017 without any issues.
It has got an IP65 protected housing, and it can measure in the temperature range from 0°C to 40°C
Can I do compliance testing with the ColiMinder?
The ColiMinder provides operational monitoring as recommended by the WHO, the EU Drinking Water Directive, and others.
Real-time measurement of current microbiological quality enables rapid response and correction, so the ColiMinder helps you to meet specified limits at the lowest cost and with the highest level of safety.
A correlation between standard lab results and ColiMinder results can be established and used internally but the system is not intended to replace compliance testing.
Can ColiMinder results be expressed in CFU ?
In contrast to classical culture-based laboratory methods, which are based on the cultivation of bacteria, the enzymatic measurement method of the ColiMinder is based on the direct measurement of the metabolic activity of the target organisms.
There is therefore no direct and generally valid conversion between the two methods.
In general, however, a correlation table can be created through comparative measurements, which can then be used for conversion.
ColiMinder device measuring E. coli levels at Blautopf, famous karstic lake in Germany
ColiMinder Emergency Response Unit (ERU) version, equipped with everytihng on board to beoperated in a car for timely scanning of different sampling points, e.g., of a recreational area
ColiMinder monitoring recreational water in the City of Paris (Canal de la Vilette).
The Coliminder in its mobile version, located on a boat, mapping E. coli contamination levels of a lake in US
ColiMinder measurements showing E.coli spread and distribution
quoted from: Stadler, P., et. al. (2019). Spatial patterns of enzymatic activity in large water bodies: Ship-borne measurements of beta-D-glucuronidase activity as a rapid indicator of microbial water quality. Science of The Total Environment, 651, 1742–1752
DOWNLOAD White paper
Several White Papers provide further in-depth information about microbiological water quality measurements with the ColiMinder.
The ColiMinder™ had proven its reliability and stability in various drinking water applications world-wide
Austrian municipal drinking water production from groundwater well: the ColiMinder monitoring raw water intake and after activated carbon filtration in alternating mode.